Heterogeneous distribution of plasma membrane glycoconjugates in pancreatic acinar cells

Oxidant-impaired intracellular Ca2+ signaling in pancreatic acinar cells: role of the plasma membrane Ca2+-ATPase.

Pancreatitis is an inflammatory disease of pancreatic acinar cells whereby intracellular calcium concentration ([Ca(2+)](i)) signaling and enzyme secretion are impaired. Increased oxidative stress has been suggested to mediate the associated cell injury. The present study tested the effects of the oxidant, hydrogen peroxide, on [Ca(2+)](i) signaling in rat pancreatic acinar cells by simultaneou...

Endocytosis at the apical plasma membrane of pancreatic acinar cells is regulated by tyrosine kinases.

We have shown that endocytosis at the apical plasma membrane of pancreatic acinar cells is regulated by the pH of the acinar lumen and is associated with cleavage of GP2, a glycosyl phosphatidylinositol-anchored protein. The aim of this study was to determine the transduction pathway by which endocytosis is activated. Apical endocytosis was studied in rat pancreatic acini by prestimulation with...

Plasma membrane internalization and recycling in rabbit lacrimal acinar cells.

PURPOSE The purpose of this study was to examine internalization and recycling of plasma membrane constituents in lacrimal gland acinar cells. METHODS Acinar cells were isolated from rabbit lacrimal glands. Surface-expressed reactive groups were biotinylated at 4 degrees C with sulfo-N-hydroxysuccinimidyl-biotin. Incorporated biotin was then labeled with avidin-horseradish peroxidase complex ...

Pancreatic Acinar Cells

Trisphosphate in Pancreatic Acinar Cells

The mechanisms of agonist-induced Ca 2 1 spikes have been investigated using a caged inositol 1,4,5-trisphosphate (IP 3 ) and a low-affinity Ca 2 1 indicator, BTC, in pancreatic acinar cells. Rapid photolysis of caged IP 3 was able to reproduce acetylcholine (ACh)induced three forms of Ca 2 1 spikes: local Ca 2 1 spikes and submicromolar ( , 1 m M) and micromolar (1–15 m M) global Ca 2 1 spikes...